3N45
Human FPPS complex with FBS_04 and zoledronic acid/MG2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 96 |
Detector technology | CCD |
Collection date | 2004-04-15 |
Detector | MARCCD165 |
Wavelength(s) | 0.97933 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 110.753, 110.753, 67.966 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.030 - 1.880 |
R-factor | 0.198 |
Rwork | 0.196 |
R-free | 0.22500 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | APRV |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.960 |
High resolution limit [Å] | 1.880 | 1.880 |
Rmerge | 0.079 | 0.405 |
Number of reflections | 34881 | 4032 |
<I/σ(I)> | 16.62 | 7.2 |
Completeness [%] | 99.8 | 100 |
Redundancy | 14.3 | 14.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 292 | 1.2M sodium potassium phosphate, 25% glycerol, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 292K |