3N2N
The Crystal Structure of Tumor Endothelial Marker 8 (TEM8) extracellular domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-28 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 65.895, 66.106, 74.439 |
| Unit cell angles | 63.68, 88.19, 59.94 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.19434 |
| Rwork | 0.192 |
| R-free | 0.22927 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1shu |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.887 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 64.680 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 86821 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.1M sodium citrate trihydrate (pH 5.6), 0.2M ammonium acetate, 20% (w/v) polyethylene glycol 4000, 0.1M hexammine cobalt (III) chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
