3N1T
Crystal structure of the H101A mutant ecHint GMP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2006-06-06 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.975 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.327, 64.066, 74.750 |
| Unit cell angles | 90.00, 109.04, 90.00 |
Refinement procedure
| Resolution | 35.330 - 1.716 |
| R-factor | 0.19644 |
| Rwork | 0.194 |
| R-free | 0.24543 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3n1s |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.029 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.700 | 1.760 |
| High resolution limit [Å] | 1.716 | 1.716 |
| Rmerge | 0.080 | 0.470 |
| Number of reflections | 41372 | |
| Completeness [%] | 88.2 | 25.8 |
| Redundancy | 3.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 40% PEG 20000, 0.1M Magnesium Acetate, 0.1M Sodium Acetate, 20mM Tris pH 7.0, 1 mM EDTA, 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
