3N0E
Crystal structure of WDR5 mutant (W330Y)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002+ |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-26 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.748, 79.680, 45.790 |
| Unit cell angles | 90.00, 101.91, 90.00 |
Refinement procedure
| Resolution | 39.050 - 2.500 |
| R-factor | 0.22243 |
| Rwork | 0.217 |
| R-free | 0.32332 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h14 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.526 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.050 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.113 | 0.158 |
| Total number of observations | 120905 | |
| Number of reflections | 10708 | |
| <I/σ(I)> | 10.6 | 7.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.81 | 6.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 18% PEG 3350, 0.1M Hepes, 14mM (NH4)2SO4, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
