3MXA

Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	72.000, 172.390, 46.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.950 - 2.300
R-factor	0.1767
Rwork	0.174
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.258
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.240	2.410
High resolution limit [Å]	2.290	2.290
Rmerge		0.498
Number of reflections	27057
<I/σ(I)>	13.4	3.7
Completeness [%]	99.9	99.5
Redundancy	6.9	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	298	20% PEG 400, 0.1M MES pH 6.5, 5% ethanol, 10% ethylene glycol, VAPOR DIFFUSION, temperature 298K