3MX6
Crystal structure of methionine aminopeptidase from Rickettsia prowazekii bound to methionine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97946 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.370, 67.550, 80.890 |
Unit cell angles | 90.00, 97.43, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.700 |
R-factor | 0.169 |
Rwork | 0.168 |
R-free | 0.20700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mr1 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.429 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.740 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.049 | 0.185 |
Number of reflections | 58037 | 3587 |
<I/σ(I)> | 21.52 | 5.9 |
Completeness [%] | 97.9 | 82.3 |
Redundancy | 4.8 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2 M sodium sulfate, 0.1 M BisTris propane, 20% PEG 3350, 20% ethylene glycol as cryo-protectant, 25.2 mg/mL protein, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |