3MX5
Lassa fever virus nucleoprotein complexed with UTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2009-01-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 3 |
| Unit cell lengths | 177.110, 177.110, 56.720 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 76.700 - 1.903 |
| R-factor | 0.18484 |
| Rwork | 0.183 |
| R-free | 0.22403 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mwp |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.493 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.560 | 1.960 |
| High resolution limit [Å] | 1.903 | 1.903 |
| Rmerge | 0.075 | 0.482 |
| Number of reflections | 79030 | |
| <I/σ(I)> | 12.6 | 3.3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.5 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 20% PEG3350, 0.2 M Lithium chloride, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 20% PEG3350, 0.2 M Lithium chloride, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
