3MV8
Crystal Structure of the TK3-Gln55His TCR in complex with HLA-B*3501/HPVG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-04 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.979457 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.760, 63.610, 88.140 |
| Unit cell angles | 101.12, 97.60, 112.00 |
Refinement procedure
| Resolution | 84.220 - 2.100 |
| R-factor | 0.231 |
| Rwork | 0.229 |
| R-free | 0.27600 |
| Structure solution method | 2fyy, 3ffc |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.046 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.200 | |
| High resolution limit [Å] | 2.100 | 10.000 | 2.100 |
| Rmerge | 0.097 | 0.030 | 0.477 |
| Number of reflections | 49428 | 466 | 6370 |
| <I/σ(I)> | 11.4 | 32 | 3 |
| Completeness [%] | 98.2 | 98.9 | 97.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 15%PEG 3350, 0.2M LiSO4, 0.1M Na-citrate, pH 5.6, vapor diffusion, hanging drop, temperature 277K |
