3MUU
Crystal structure of the Sindbis virus E2-E1 heterodimer at low pH
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-31 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97934,0.97951,0.94934 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.482, 158.430, 160.676 |
| Unit cell angles | 60.42, 89.80, 89.65 |
Refinement procedure
| Resolution | 60.720 - 3.290 |
| R-factor | 0.24 |
| Rwork | 0.239 |
| R-free | 0.25200 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.235 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 3.360 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.128 | 0.658 |
| Number of reflections | 162989 | |
| <I/σ(I)> | 12.5 | 1.8 |
| Completeness [%] | 97.3 | 82.9 |
| Redundancy | 3.8 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 293 | PEG8000 10%, Sodium/Potassium Tartrate 325 mM, Sodium/Potassium Phosphate 100 mM pH5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
