3MU3
Crystal structure of chicken MD-1 complexed with lipid IVa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0333 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.465, 78.150, 101.236 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.23388 |
| Rwork | 0.232 |
| R-free | 0.26703 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 3mtx |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.686 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.044 | 0.371 |
| Number of reflections | 12503 | |
| <I/σ(I)> | 6.6 | |
| Completeness [%] | 98.2 | |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 296 | 17% PEG 2000, 100mM MES pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
