3MTC
Crystal Structure of INPP5B in complex with phosphatidylinositol 4-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97908 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 133.761, 133.761, 133.761 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.290 - 2.400 |
| R-factor | 0.17234 |
| Rwork | 0.171 |
| R-free | 0.20544 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i9y |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.278 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.510 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.091 | 0.698 |
| Number of reflections | 31380 | |
| <I/σ(I)> | 11.1 | 2 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 4.8 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 22% polyacrylic acid, 0.02M magnesium chloride, 0.1M hepes pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
