3MSX
Crystal structure of RhoA.GDP.MgF3 in complex with GAP domain of ArhGAP20
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-04 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97166 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 69.057, 88.977, 137.725 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.230 - 1.650 |
R-factor | 0.196 |
Rwork | 0.195 |
R-free | 0.21900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.503 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 1.689 |
High resolution limit [Å] | 1.650 | 6.460 | 1.650 |
Rmerge | 0.061 | 0.055 | 0.120 |
Number of reflections | 41890 | ||
<I/σ(I)> | 12.3 | ||
Completeness [%] | 99.9 | 99.6 | 100 |
Redundancy | 7.2 | 6.6 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Crystallization drop was 1:1 mixture of protein dissolved in 20 mM Tris, pH 8.5, 100 mM NaCl, 5 mM NaF, 5 mM MgCl2, 5mM 2-mercaptoethanol and 20 w/v% PEG 8000, 0.2 M NaCl, 0.17 M NH4Cl, 0.1 M phosphate citrate buffer. The crystallization reservoir was 20 w/v% PEG 8000, 0.2 M NaCl, 0.17 M NH4Cl, 0.1 M phosphate citrate buffer, vapor diffusion, sitting drop, temperature 293K |