3MP7

Lateral opening of a translocon upon entry of protein suggests the mechanism of insertion into membranes

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	77
Detector technology	CCD
Collection date	2008-12-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97966, 0.97958, 0.96863
Spacegroup name	C 2 2 21
Unit cell lengths	71.240, 141.570, 235.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.430 - 2.900
R-factor	0.28
Rwork	0.277
R-free	0.31700
Structure solution method	MAD
Starting model (for MR)	1rh5
RMSD bond length	0.010
RMSD bond angle	1.300
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	ELVES

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.900
Number of reflections	23475
<I/σ(I)>	9.8
Completeness [%]	98.0
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	273	PEG4000-PEG8000 15-25%, MES 100mM pH 6.2, Ca or Mg Acetate 50-200mM, VAPOR DIFFUSION, HANGING DROP, temperature 273K
1	VAPOR DIFFUSION, HANGING DROP	6.2	273	PEG4000-PEG8000 15-25%, MES 100mM pH 6.2, Ca or Mg Acetate 50-200mM, VAPOR DIFFUSION, HANGING DROP, temperature 273K