3MOR
Crystal structure of Cathepsin B from Trypanosoma Brucei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.910, 75.490, 75.600 |
| Unit cell angles | 90.00, 104.80, 90.00 |
Refinement procedure
| Resolution | 48.000 - 2.550 |
| R-factor | 0.20445 |
| Rwork | 0.202 |
| R-free | 0.24299 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qdq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.600 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.670 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Number of reflections | 34696 | |
| <I/σ(I)> | 6.99 | |
| Completeness [%] | 97.8 | 97.4 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | PEG 3350,(NH4)2HPO4, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
