3MO2
human G9a-like (GLP, also known as EHMT1) in complex with inhibitor E67
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-14 |
Detector | MARMOSAIC 300 mm CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.700, 163.000, 69.300 |
Unit cell angles | 90.00, 98.90, 90.00 |
Refinement procedure
Resolution | 31.830 - 2.490 |
R-factor | 0.192 |
Rwork | 0.192 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fpd |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.830 | 2.580 |
High resolution limit [Å] | 2.490 | 2.490 |
Rmerge | 0.095 | 0.384 |
Number of reflections | 43245 | |
<I/σ(I)> | 12.8 | 2.6 |
Completeness [%] | 94.4 | 94.3 |
Redundancy | 4.3 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1 M Hepes pH 7.5, 14% polyethylene glycol 4000, 9% isopropanol, and 12% dimethyl sulfoxide, VAPOR DIFFUSION, HANGING DROP, temperature 289K |