3MJ0
Crystal Structure of Drosophia Ago-PAZ domain in complex with 3'-end 2'-O-methylated RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-26 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97929 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 69.211, 116.796, 38.661 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.785 - 2.306 |
| R-factor | 0.2244 |
| Rwork | 0.222 |
| R-free | 0.27430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r6z |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.502 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.045 | 0.327 |
| Number of reflections | 7212 | |
| <I/σ(I)> | 35.04 | 5.578 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 6.8 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 293 | 50 mM MES, pH 5.8, 100 mM ammonium sulfate, 10 mM MgCl2, 16% PEG 8000 and 5% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
