3MHR
14-3-3 sigma in complex with YAP pS127-peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.85 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 82.270, 112.110, 62.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.620 - 1.150 |
| R-factor | 0.12779 |
| Rwork | 0.127 |
| R-free | 0.15298 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lw1 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.055 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.620 | 1.200 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.080 | 0.351 |
| Number of reflections | 102833 | |
| <I/σ(I)> | 16.54 | 5.23 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 5.31 | 4.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 277 | 0.095M HEPES Na-Salt pH7.4, 25.6% PEG 400, 0.19M CaCl2, 5% Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
