3MGS
Binding of Cesium ions to the Nucleosome Core particle
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-22 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.93 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 106.510, 109.668, 182.609 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 94.070 - 3.150 |
R-factor | 0.21635 |
Rwork | 0.216 |
R-free | 0.23756 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kx5 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.460 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | REFMAC (Rigid Body) |
Refinement software | REFMAC (Rigid Body) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 94.070 | 3.320 |
High resolution limit [Å] | 3.150 | 3.150 |
Rmerge | 0.091 | 0.477 |
Number of reflections | 36839 | |
<I/σ(I)> | 17.8 | 3.1 |
Completeness [%] | 99.8 | 98.5 |
Redundancy | 7 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 291 | 85mM MnCl2, 60mM KCl, 40mM K-cacodylate , pH 6.0, VAPOR DIFFUSION, temperature 291K |