3MGQ
Binding of Nickel ions to the Nucleosome Core Particle
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2009-06-22 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.48 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 106.129, 109.643, 183.109 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.000 - 2.650 |
R-factor | 0.23319 |
Rwork | 0.232 |
R-free | 0.27613 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kx5 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.498 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | REFMAC (Rigid Body) |
Refinement software | REFMAC (Rigid Body) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 94.070 | 2.790 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.064 | 0.496 |
Number of reflections | 60265 | |
<I/σ(I)> | 19.7 | 2 |
Completeness [%] | 98.2 | 89.2 |
Redundancy | 5.6 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 291 | 85mM MnCl2, 60mM KCl, 40mM K-cacodylate , pH 6.0, VAPOR DIFFUSION, temperature 291K |