3MGP
Binding of Cobalt ions to the Nucleosome Core Particle
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-06-22 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.6 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 106.502, 109.940, 183.352 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.600 - 2.440 |
| R-factor | 0.24022 |
| Rwork | 0.239 |
| R-free | 0.28245 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kx5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.433 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC (Rigid Body) |
| Refinement software | REFMAC (Rigid Body) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.490 | 2.570 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.063 | 0.442 |
| Number of reflections | 72231 | |
| <I/σ(I)> | 17 | 1 |
| Completeness [%] | 91.6 | 59.5 |
| Redundancy | 4.7 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 291 | 85mM MnCl2, 60mM KCl, 40mM K-cacodylate, pH 6.0, VAPOR DIFFUSION, temperature 291K |
