3MGP
Binding of Cobalt ions to the Nucleosome Core Particle
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2009-06-22 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.6 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 106.502, 109.940, 183.352 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.600 - 2.440 |
R-factor | 0.24022 |
Rwork | 0.239 |
R-free | 0.28245 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kx5 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.433 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | REFMAC (Rigid Body) |
Refinement software | REFMAC (Rigid Body) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 94.490 | 2.570 |
High resolution limit [Å] | 2.440 | 2.440 |
Rmerge | 0.063 | 0.442 |
Number of reflections | 72231 | |
<I/σ(I)> | 17 | 1 |
Completeness [%] | 91.6 | 59.5 |
Redundancy | 4.7 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 291 | 85mM MnCl2, 60mM KCl, 40mM K-cacodylate, pH 6.0, VAPOR DIFFUSION, temperature 291K |