3MEV
Crystal structure of SGF29 in complex with R2AK4me3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.966, 64.979, 104.967 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.040 - 1.830 |
R-factor | 0.23014 |
Rwork | 0.227 |
R-free | 0.28213 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.411 |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.250 | 1.860 |
High resolution limit [Å] | 1.830 | 1.830 |
Number of reflections | 29380 | |
<I/σ(I)> | 12.6 | 5.96 |
Completeness [%] | 95.2 | 88.9 |
Redundancy | 7.2 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 20-28% PEG3350, 0.1M Bis-Tris pH 5.5, vapor diffusion, Sitting drop, temperature 291K, VAPOR DIFFUSION, SITTING DROP |