3MDJ
ER Aminopeptidase, ERAP1, Bound to the Zinc Aminopeptidase Inhibitor, Bestatin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Detector technology | CCD |
Collection date | 2008-06-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0809 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 71.029, 234.635, 95.860 |
Unit cell angles | 90.00, 103.59, 90.00 |
Refinement procedure
Resolution | 38.106 - 2.950 |
R-factor | 0.202 |
Rwork | 0.199 |
R-free | 0.26420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1z5h |
RMSD bond length | 0.005 |
RMSD bond angle | 0.850 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.5_2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.060 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 0.162 | |
Number of reflections | 63768 | |
<I/σ(I)> | 5.1 | 1.1 |
Completeness [%] | 99.8 | 98.8 |
Redundancy | 3.6 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 14% PEG 8000, 0.1M Bicine pH 8.5, 1mM Glutathione, VAPOR DIFFUSION, HANGING DROP, temperature 277K |