3MCA

Structure of the Dom34-Hbs1 Complex and implications for its role in No-Go decay

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-12
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9798
Spacegroup name	P 21 21 21
Unit cell lengths	76.741, 113.423, 124.742
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.740
R-factor	0.2486
Rwork	0.247
R-free	0.28600
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.493
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	83.919	113.423	2.640
High resolution limit [Å]	2.600	14.240	2.600
Rmerge		0.028	0.933
Rmeas	0.084	0.042	1.065
Rpim	0.030	0.016	0.370
Total number of observations	270895	1455	13753
Number of reflections	34234
<I/σ(I)>	18.7	49.2	2.1
Completeness [%]	99.9	95.6	100
Redundancy	7.9	5.9	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	6% PEG 8000, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K