3MBG
Crystal Structure of Human Augmenter of Liver Regeneration (ALR)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-08-13 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.719, 65.145, 63.767 |
| Unit cell angles | 90.00, 89.97, 90.00 |
Refinement procedure
| Resolution | 27.760 - 1.850 |
| R-factor | 0.19129 |
| Rwork | 0.189 |
| R-free | 0.23121 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oqc |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.889 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.760 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.044 | 0.366 |
| Number of reflections | 49190 | |
| <I/σ(I)> | 36.54 | 3.13 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 18% w/v PEG 8000, 210 mM zinc acetate dihydrate, 100 mM sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
