3MAV
Crystal structure of Plasmodium vivax putative farnesyl pyrophosphate synthase (Pv092040)
Replaces: 2IHIExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-09-12 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.969, 116.390, 92.416 |
| Unit cell angles | 90.00, 116.01, 90.00 |
Refinement procedure
| Resolution | 20.990 - 2.100 |
| R-factor | 0.2291 |
| Rwork | 0.229 |
| R-free | 0.26150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yhk |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.920 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.054 | 0.564 |
| Number of reflections | 92263 | |
| <I/σ(I)> | 17.5 | 2.53 |
| Completeness [%] | 99.1 | 96.6 |
| Redundancy | 4.7 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 297 | 22% PEG 3350, 200 MM LI2SO4, 100 MM TRIS pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
