3M85
Archaeoglobus fulgidus exosome y70a with RNA bound to the active site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-09-06 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.006 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 138.020, 138.020, 262.250 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.984 - 3.000 |
R-factor | 0.1995 |
Rwork | 0.191 |
R-free | 0.27780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ab1 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.256 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.4_129)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.100 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 51203 | |
Completeness [%] | 98.9 | 98.9 |
Redundancy | 7.22 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.1M Na Acetate, pH 4.6, 30% MPD, 100mM NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 293K |