3M84
Crystal Structure of Phosphoribosylaminoimidazole Synthetase from Francisella tularensis
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-21 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.97915 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.708, 90.560, 118.989 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.751 - 1.699 |
R-factor | 0.155 |
Rwork | 0.154 |
R-free | 0.18300 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.208 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.730 |
High resolution limit [Å] | 1.699 | 1.699 |
Number of reflections | 72851 | |
<I/σ(I)> | 18.3 | 4.3 |
Completeness [%] | 96.7 | 75.3 |
Redundancy | 3.6 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.2M Litium Sulfate; 0.1M Tris pH 7.0; 2M Ammonium Sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 289K |