3M7F

Crystal structure of the Nedd4 C2/Grb10 SH2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-28
Detector	ADSC QUANTUM 210
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	52.163, 70.086, 86.246
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.123 - 2.000
R-factor	0.1945
Rwork	0.193
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1NRV AND 3B7Y
RMSD bond length	0.006
RMSD bond angle	1.057
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Rmerge	0.052
Number of reflections	21866
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.5	295	crystallization in 35% MPD, pH 6.5, EVAPORATION, temperature 295K