3M6V

Multi-site-specific 16S rRNA methyltransferase RsmF from Thermus thermophilus in space group P2 in complex with S-Adenosyl-L-Methionine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4C
Synchrotron site	NSLS
Beamline	X4C
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-03
Detector	MAR CCD 165 mm
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	65.956, 78.316, 108.122
Unit cell angles	90.00, 107.05, 90.00

Refinement procedure

Resolution	29.068 - 1.820
R-factor	0.164
Rwork	0.162
R-free	0.19400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3m6u
RMSD bond length	0.006
RMSD bond angle	1.038
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.5_2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.890
High resolution limit [Å]	1.820	3.920	1.820
Rmerge	0.082	0.055	0.380
Number of reflections	93014
<I/σ(I)>	8.5
Completeness [%]	97.0	95.3	86.5
Redundancy	2.8	3	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	277	200 mM NaCl, 12% w/v PEG8000 and 100 mM HEPES-KOH (pH7.5), microbatch under oil, temperature 277K