3M61
Crystal structure of complex of urokinase and a upain-1 variant(W3A) in pH4.6 condition
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-23 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 |
Unit cell lengths | 121.705, 121.705, 43.128 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.130 - 1.680 |
R-factor | 0.19773 |
Rwork | 0.195 |
R-free | 0.24309 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nwn |
RMSD bond length | 0.013 |
RMSD bond angle | 1.439 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.710 |
High resolution limit [Å] | 1.650 | 1.680 |
Rmerge | 0.117 | 0.487 |
Number of reflections | 22669 | |
<I/σ(I)> | 10.8 | 1.03 |
Completeness [%] | 88.4 | 89.1 |
Redundancy | 3.1 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 2.0M ammonium sulfate, 50mM sodium citrate pH 4.60, 5% PEG 400, VAPOR DIFFUSION, SITTING DROP, temperature 298K |