3M55

SET7/9 Y305F in complex with TAF10-K189me1 peptide and AdoHcy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-13
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9786
Spacegroup name	P 32 2 1
Unit cell lengths	83.524, 83.524, 95.673
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.190 - 1.550
R-factor	0.192
Rwork	0.191
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2f69
RMSD bond length	0.012
RMSD bond angle	1.391
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.610
High resolution limit [Å]	1.550	3.340	1.550
Rmerge	0.053	0.036	0.388
Number of reflections	56257
<I/σ(I)>	15.2
Completeness [%]	99.1	99.6	91.8
Redundancy	10.2	10.6	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.3	293	0.95 M Sodium Citrate, 0.1 M Imidazole pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K