3M2M
Rat galectin-1 complex with lactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1993-11-02 |
| Detector | SIEMENS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.100, 58.600, 121.600 |
| Unit cell angles | 101.10, 91.60, 110.90 |
Refinement procedure
| Resolution | 36.000 - 2.950 |
| R-factor | 0.233 |
| Rwork | 0.206 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.330 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MERLOT |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | 3.080 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Number of reflections | 15682 | |
| Completeness [%] | 73.7 | 42.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 30mM NaOAc, PEG 4K 15-17%, 4mM 2-mercaptoethanol, 100mM lactose, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
