3M1N
Crystal structure of Human Sonic Hedgehog N-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-01-01 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.741, 51.788, 69.932 |
| Unit cell angles | 90.00, 96.53, 90.00 |
Refinement procedure
| Resolution | 18.730 - 1.850 |
| R-factor | 0.20574 |
| Rwork | 0.203 |
| R-free | 0.25958 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vhh |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.802 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.066 | 0.302 |
| Number of reflections | 27477 | |
| <I/σ(I)> | 2.77 | |
| Completeness [%] | 98.3 | 91 |
| Redundancy | 3.3 | 2.31 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 273 | 21-24% PEG 4000, 200 mM LiSO4, and 100 mM Tris, pH = 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
