3LYF
Crystal Structure of the Rift Valley Fever Virus Nucleocapsid Protein
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-03 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9794,0.9796 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.110, 69.630, 80.620 |
| Unit cell angles | 78.45, 69.72, 60.97 |
Refinement procedure
| Resolution | 46.510 - 1.930 |
| R-factor | 0.21361 |
| Rwork | 0.211 |
| R-free | 0.25428 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.293 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.080 | 0.430 |
| Number of reflections | 87736 | |
| <I/σ(I)> | 9.6 | 3.1 |
| Completeness [%] | 97.1 | 95.7 |
| Redundancy | 4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277.15 | 26% PEG 3350, 100 mM Na/K phosphate pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277.15 | 26% PEG 3350, 100 mM Na/K phosphate pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
