3LYF
Crystal Structure of the Rift Valley Fever Virus Nucleocapsid Protein
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-03 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9794,0.9796 |
Spacegroup name | P 1 |
Unit cell lengths | 67.110, 69.630, 80.620 |
Unit cell angles | 78.45, 69.72, 60.97 |
Refinement procedure
Resolution | 46.510 - 1.930 |
R-factor | 0.21361 |
Rwork | 0.211 |
R-free | 0.25428 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.293 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 1.930 | 1.930 |
Rmerge | 0.080 | 0.430 |
Number of reflections | 87736 | |
<I/σ(I)> | 9.6 | 3.1 |
Completeness [%] | 97.1 | 95.7 |
Redundancy | 4 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277.15 | 26% PEG 3350, 100 mM Na/K phosphate pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277.15 | 26% PEG 3350, 100 mM Na/K phosphate pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |