3LY6
Crystal structure of human transglutaminase 2 complex with adenosine 5' Triphosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 6C1 |
Synchrotron site | PAL/PLS |
Beamline | 6C1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-10 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 1 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 134.175, 215.759, 165.956 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.140 |
R-factor | 0.2333 |
Rwork | 0.233 |
R-free | 0.29690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kv3 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.260 |
High resolution limit [Å] | 3.140 | 3.140 |
Number of reflections | 41905 | |
Completeness [%] | 99.2 | 98.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 277 | 50MM MES, 200MM NACL, 50MM MGCL2, 6-8% PEG3350,5MM DTT, 20% GLYCEROL, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |