3LXA
Interconversion of Human Lysosomal Enzyme Specificities
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98010 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.241, 104.959, 181.038 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.630 - 3.040 |
R-factor | 0.216 |
Rwork | 0.214 |
R-free | 0.24400 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3hg3 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.413 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.271 | 0.851 |
Number of reflections | 23638 | |
<I/σ(I)> | 7.6 | 1.8 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 7.3 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG8000, Sodium Cacodylate, Magnesium Acetate, pH 6.5, vapor diffusion, hanging drop, temperature 293K |