3LWW
Structure of an open and closed conformation of Human Importin Beta bound to the Snurportin1 IBB-domain trapped in the same crystallographic asymmetric unit
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-28 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 101.650, 101.735, 101.963 |
Unit cell angles | 90.00, 110.88, 90.00 |
Refinement procedure
Resolution | 14.957 - 3.150 |
R-factor | 0.2663 |
Rwork | 0.264 |
R-free | 0.31290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID: 2P8Q |
RMSD bond length | 0.003 |
RMSD bond angle | 0.716 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | |
High resolution limit [Å] | 3.150 | 3.150 |
Number of reflections | 32721 | |
<I/σ(I)> | 16.3 | |
Completeness [%] | 99.2 | 99.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 18% PEG 8000, 50 mM sodium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 18% PEG 8000, 50 mM sodium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |