3LVW
Glutathione-inhibited ScGCL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-11-12 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 118.127, 118.127, 165.799 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.880 - 2.500 |
R-factor | 0.20171 |
Rwork | 0.199 |
R-free | 0.25054 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ig5 |
RMSD bond length | 0.021 |
RMSD bond angle | 1.926 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.101 | 0.533 |
Number of reflections | 40046 | |
<I/σ(I)> | 9.7 | 3 |
Completeness [%] | 97.1 | 96.1 |
Redundancy | 8.77 | 8.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 291 | 100mM Hepes, pH 6.8, 14-20% PEG 400, 5mM GSH, 5mM MgCl2 , VAPOR DIFFUSION, SITTING DROP, temperature 291K |