3LVL
Crystal Structure of E.coli IscS-IscU complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | OTHER |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-21 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9793 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 77.647, 77.647, 355.986 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.489 - 3.000 |
R-factor | 0.227 |
Rwork | 0.225 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1P3W and 2Z7E |
RMSD bond length | 0.004 |
RMSD bond angle | 0.654 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.6_289) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
Rmerge | 0.093 | 0.053 | 0.285 |
Number of reflections | 11488 | ||
<I/σ(I)> | 12.1 | ||
Completeness [%] | 83.4 | 96.8 | 36.5 |
Redundancy | 16.6 | 18 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 295 | 16% PEG 8000, 0.2M Sodium Nitrate, 4% Glycerol, 0.1M Bicine pH 9.0, vapor diffusion, sitting drop, temperature 295K |