3LUM
Structure of ulilysin mutant M290L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Detector technology | CCD |
| Collection date | 2009-02-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.976 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 97.310, 174.110, 124.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.280 - 1.700 |
| R-factor | 0.16289 |
| Rwork | 0.163 |
| R-free | 0.19487 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cki |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.356 |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.700 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.104 | 0.196 |
| Number of reflections | 115779 | |
| <I/σ(I)> | 15.2 | 7.9 |
| Redundancy | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | pH 7.5, VAPOR DIFFUSION, SITTING DROP |
