3LUK

Crystal structure of MID domain from hAGO2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	200
Detector technology	CCD
Collection date	2009-10-10
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9785
Spacegroup name	P 1
Unit cell lengths	40.800, 47.851, 66.846
Unit cell angles	85.90, 72.84, 82.86

Refinement procedure

Resolution	30.000 - 1.700
R-factor	0.1909
Rwork	0.188
R-free	0.23190
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.982
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.914	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	46880
Completeness [%]	100.0	58.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	0.1 M imidazole, 0.2 M NaCl, 0.46 M NaH2PO4, 1.84 M K2HPO4. Protein: 15 mg/mL, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K