3LRN
Crystal structure of human RIG-I CTD bound to a 14 bp GC 5' ppp dsRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 174 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-09-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.490, 96.330, 103.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.500 - 2.600 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | free human RIG-I CTD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.680 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.086 | 0.587 |
| Number of reflections | 13150 | |
| <I/σ(I)> | 27.4 | 3.2 |
| Completeness [%] | 98.7 | 96.3 |
| Redundancy | 4.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 15% PEG 3,350, ammonium sulfate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG | ||
| 2 | 1 | 1 | ammonium sulfate | ||
| 3 | 1 | 2 | PEG | ||
| 4 | 1 | 2 | ammonium sulfate |
