3LRN

Crystal structure of human RIG-I CTD bound to a 14 bp GC 5' ppp dsRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	174
Detector technology	IMAGE PLATE
Collection date	2009-09-01
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.542
Spacegroup name	P 21 21 21
Unit cell lengths	41.490, 96.330, 103.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.500 - 2.600
R-factor	0.221
Rwork	0.221
R-free	0.28200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	free human RIG-I CTD
RMSD bond length	0.013
RMSD bond angle	1.680
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.086	0.587
Number of reflections	13150
<I/σ(I)>	27.4	3.2
Completeness [%]	98.7	96.3
Redundancy	4.5	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	277	15% PEG 3,350, ammonium sulfate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG
2	1	1	ammonium sulfate
3	1	2	PEG
4	1	2	ammonium sulfate