3LRL
Structure of alfa-galactosidase (MEL1) from Saccharomyces cerevisiae with melibiose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 100.729, 100.729, 111.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.990 - 2.400 |
| R-factor | 0.20131 |
| Rwork | 0.198 |
| R-free | 0.25336 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uas |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.546 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 111.280 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.111 | 0.433 |
| Number of reflections | 22940 | |
| <I/σ(I)> | 6.7 | 5.8 |
| Completeness [%] | 99.6 | 99.2 |
| Redundancy | 10.4 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | Deglycosylated sample. 19% PEG3350, 0.1 M BisTris, 0.2 M SCNK, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
