3LRG
Structure of anti-huntingtin VL domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-01-13 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 107.680, 107.680, 50.620 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.050 |
R-factor | 0.17181 |
Rwork | 0.170 |
R-free | 0.19669 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mfa |
RMSD bond length | 0.015 |
RMSD bond angle | 1.373 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.100 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.072 | 0.264 |
Number of reflections | 19201 | |
<I/σ(I)> | 32.7 | 11.7 |
Completeness [%] | 99.8 | 100 |
Redundancy | 15 | 14.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.8 | 293 | 1.2 M Ammonium sulfate, 0.2 M Sodium chloride, 0.1 M CHES-NAOH, pH 9.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |