Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 81.880, 111.670, 113.760 |
| Unit cell angles | 80.03, 74.34, 68.83 |
Refinement procedure
| Resolution | 30.000 - 4.004 |
| R-factor | 0.32 |
| Rwork | 0.319 |
| R-free | 0.33120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3H5M |
| RMSD bond length | 0.032 |
| RMSD bond angle | 1.731 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 4.200 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.042 | 0.153 |
| Number of reflections | 58157 | |
| <I/σ(I)> | 1.48 | |
| Completeness [%] | 95.6 | 94.8 |
| Redundancy | 1.1 | 1.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 20% PEG 400, 100mM Tris pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
