Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 92.810, 108.300, 138.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.323 - 3.610 |
| R-factor | 0.2965 |
| Rwork | 0.295 |
| R-free | 0.31840 |
| Structure solution method | SAD |
| RMSD bond length | 0.031 |
| RMSD bond angle | 1.857 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.750 |
| High resolution limit [Å] | 3.610 | 3.610 |
| Rmerge | 0.083 | 0.463 |
| Number of reflections | 16598 | |
| Completeness [%] | 99.9 | |
| Redundancy | 10.8 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.4% NG, 0.1mM Tris, 22% (w/v) PEG 400, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K , temperature 291.0K |
