3LQX
SRP ribonucleoprotein core complexed with cobalt hexammine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 136.818, 77.765, 32.735 |
Unit cell angles | 90.00, 94.08, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.930 |
Rwork | 0.244 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dul |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 1.930 |
Number of reflections | 47287 |
Completeness [%] | 93.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 50 mM Na-MES, 200 mM potassium acetate, 5 mM cobalt hexammine, 10% isopropanol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |