3LQ9
Crystal structure of human REDD1, a hypoxia-induced regulator of mTOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-01 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.331, 36.586, 47.970 |
| Unit cell angles | 77.56, 89.08, 86.18 |
Refinement procedure
| Resolution | 27.096 - 2.000 |
| R-factor | 0.179 |
| Rwork | 0.177 |
| R-free | 0.21400 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.770 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.068 | 0.045 | 0.259 |
| Number of reflections | 14319 | ||
| <I/σ(I)> | 15.9 | ||
| Completeness [%] | 95.9 | 97 | 92.5 |
| Redundancy | 4 | 4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M NaF, 20-26% PEG3350, 0.05 mM C12E9, vapor diffusion, hanging drop, temperature 277K | |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M NaF, 20-26% PEG3350, 0.05 mM C12E9, vapor diffusion, hanging drop, temperature 277K |
