3LP4
Crystal structure of human arginase I in complex with L-LYSINE, 1.90A Resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Detector technology | CCD |
| Collection date | 2008-12-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 3 |
| Unit cell lengths | 90.729, 90.729, 69.509 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.200 - 1.900 |
| Rwork | 0.155 |
| R-free | 0.20380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zav |
| RMSD bond angle | 0.006 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.081 | 0.433 |
| Number of reflections | 49010 | |
| <I/σ(I)> | 17.4 | 2.5 |
| Completeness [%] | 97.1 | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 0.1 M bis-Tris pH 6.5, 28% PEGMME 2000. Crystals were harvested and soaked in a precipitant solution augmented with 20 mM L-lysine. Cryoprotected in 32% Jeffamine, VAPOR DIFFUSION, HANGING DROP |
