3LNJ
Crystal structure of human MDM2 in complex with D-peptide inhibitor (DPMI-alpha)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-08-20 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 68.976, 213.478, 45.536 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 106.740 - 2.400 |
| R-factor | 0.21085 |
| Rwork | 0.209 |
| R-free | 0.25494 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eqs |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.989 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 106.740 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.065 | 0.775 |
| Number of reflections | 13268 | |
| <I/σ(I)> | 29.5 | 2 |
| Completeness [%] | 96.0 | 97.6 |
| Redundancy | 6.2 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 273 | 0.2 M ammonium sulfate, 0.1 M sodium cacodylate trihydrate, and 30% PEG 8000, pH 6.5., VAPOR DIFFUSION, HANGING DROP, temperature 273K |
